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目的探讨西北部分地区无偿献血人群中丙型肝炎病毒(HCV)基因型(亚型)的分布。方法收集无偿献血人群中HCV RNA阳性标本,通过RT-PCR扩增NS5B基因,测定其核苷酸序列后,与Genebank中已知序列进行分子进化分析,确定HCV基因型(亚型)。结果对西北部分地区(新疆、陕西、青海)137例HCV RNA阳性血清进行NS5B基因扩增,扩增阳性128例,其中1b占43.00%(55/128),2a占35.16%(45/128),3a占9.38%(12/128),3b占10.16%(13/128),6a占2.34%(3/128)。1b亚型之间同源性最高为99%,最低为93%,2a亚型之间同源性最高为98%,最低为91%,3a亚型之间同源性最高为98%,最低为91%,3b亚型之间同源性最高为98%,最低为93%。结论西北部分地区无偿献血人群中HCV基因分型以1b和2a为主,3a,3b,6a亚型占的比例较低,和中国内陆地区的基因型分布相似。
Objective To investigate the distribution of hepatitis C virus (HCV) genotypes in sub-national blood donors in Northwest China. Methods The HCV RNA positive samples were collected from unrelated blood donors. The NS5B gene was amplified by RT-PCR. The nucleotide sequence of the NS5B gene was determined by RT-PCR. The genotypes of the HCV genotypes (subtypes) were determined by molecular phylogenetic analysis. Results 137 cases of HCV RNA positive sera from northwestern part of China (Xinjiang, Shaanxi and Qinghai) were amplified by NS5B gene. 128 cases were positive, of which 1b accounted for 43.00% (55/128), 2a accounted for 35.16% (45/128) , 3a accounted for 9.38% (12/128), 3b accounted for 10.16% (13/128), and 6a accounted for 2.34% (3/128). The homology of subtype 1b was 99%, the lowest was 93%. The homology between subtypes 2a was 98%, the lowest was 91%, and the highest homology was 98% between 3a subtypes 91%. The highest homology between subtypes 3b is 98% and the lowest is 93%. Conclusion HCV genotypes are mainly 1b and 2a in unpaid blood donors in some areas of Northwest China. The proportion of HCV genotypes 3a, 3b and 6a is lower, which is similar to that of inland China.