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目的 研究转移抑制基因(nm23)和基质金属蛋白酶-7(MMP-7)在子宫内膜癌前病变及子宫内膜样腺癌组织中的异常表达及变化规律,探讨其作为子宫内膜早期癌变及浸润、转移的生物学标志的可能性。方法 应用免疫组化S-P法对15例正常增生期子宫内膜组织、40例子宫内膜癌前病变组织、38 例子宫内膜样腺癌组织中nm23、MMP-7蛋白的表达进行研究。结果 在正常增生期子宫内膜、子宫内膜简单型增生、复杂型增生、不典型增生、子宫内膜样腺癌(包括浸润深度≤1/2肌层、>1/2 肌层),nm23 呈递减趋势,MMP-7 蛋白的阳性表达率呈递增趋势。等级相关分析结果显示:nm23、MMP-7表达异常与癌组织学分级均显著相关(相关系数r分别为-0. 532和0. 410,P值均<0 .01)。除不典型增生外,正常增生期子宫内膜、子宫内膜简单型增生、复杂型增生与子宫内膜样腺癌组织中的nm23、MMP-7蛋白表达差异有显著性。正常增生期子宫内膜、子宫内膜简单型增生与不典型增生比较,nm23、MMP-7 蛋白表达有显著差异;在内膜样腺癌中,MMP-7、nm23 表达存在负相关性(r =-0 .392 ,P<0 .01);MMP-7与肿瘤浸润深度、临床分期呈正相关(r=0.511,P<0.01);nm23蛋白表达与子宫内膜样腺癌的手术分期、组织学分级、肌层浸润无关(P>0 .05)。结论 nm23 蛋白、MMP-7 在子宫内
Objective To investigate the abnormal expression and changes of metastasis suppressor gene (nm23) and matrix metalloproteinase-7 (MMP-7) in endometrial precancerous lesions and endometrioid adenocarcinoma and to investigate its role as early endometrial cancer And invasive, metastatic biological markers. Methods The expressions of nm23 and MMP-7 in 15 cases of normal endometrial carcinoma, 40 cases of endometrial precancerous lesions and 38 cases of endometrial adenocarcinoma were studied by immunohistochemical S-P method. Results In the normal proliferative endometrium, endometrial simple hyperplasia, complex hyperplasia, dysplasia, endometrioid adenocarcinoma (including infiltration depth less than 1/2 myometrium,> 1/2 myometrium), nm23 Showed a decreasing trend, the positive expression rate of MMP-7 protein showed an increasing trend. The results of rank correlation analysis showed that the expression of nm23 and MMP-7 were significantly correlated with the histological grade (r = -0.532 and -0.41, respectively, P <0.01). In addition to dysplasia, normal proliferative endometrium, simple endometrial hyperplasia, complex hyperplasia and endometrial adenocarcinoma of nm23, MMP-7 protein expression was significantly different. The expressions of nm23 and MMP-7 in normal proliferative endometrium, endometrial simple hyperplasia and atypical hyperplasia were significantly different. In endometrial adenocarcinoma, there was a negative correlation between the expression of MMP-7 and nm23 (r = -0.392, P <0.01). There was a positive correlation between the expression of MMP-7 and tumor invasion depth and clinical stage (r = 0.511, P <0.01) Learning grade, muscle invasion has nothing to do (P> 0.05). Conclusion The nm23 protein and MMP-7 are in the uterus