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利用人工繁育技术对虹鳟(♀)(Oncorhynchus mykiss)和山女鳟(♂)(Oncorhynchus masou masou)进行了种间杂交实验,并对杂交胚胎和仔鱼的发育进行了观察,通过观察,详细描述了其胚胎发育和仔鱼发育各个阶段的发育时序和形态特征。结果表明:在水温为4.3-9.2℃的条件下,杂交受精卵的有效发育积温达到206.01℃·d发眼,完成胚胎发育所需的有效积温为387.44℃·d,杂交鳟的胚胎发育速度介于双亲之间;初孵仔鱼,全身透明,血液循环和心跳清晰可见,肛突明显,背鳍原基出现;破膜后20d,全长为21.25±1.40mm,此时约半数卵黄囊体积明显减小,可以看见背鳍、臀鳍与尾鳍的出现;破膜后40d,个别发育较快的个体开始在水中游动,主动摄食;破膜后45d,卵黄囊基本吸收完全,仔鱼具有游泳能力,50d后几乎全部上浮。
Interspecific hybridization experiments were carried out on Oncorhynchus mykiss and Oncorhynchus masou masou using artificial breeding techniques. The development of hybrid embryos and larvae was observed and the results were described in detail Its embryonic development and larval development stages of developmental timing and morphological characteristics. The results showed that when the water temperature was 4.3-9.2 ℃, the accumulated effective temperature reached to 206.01 ℃ · d, the effective accumulated temperature to complete embryo development was 387.44 ℃ · d, the embryo development speed of hybrid trout Between the parents; newly hatched larvae, body transparent, blood circulation and heartbeat clearly visible anus prominent, dorsal fin primordia; 20d after rupture of the membrane, the total length of 21.25 ± 1.40mm, at this time about half the volume of yolk sac significantly reduced Small, you can see the dorsal fin, anal fin and caudal fin appeared; 40d after the rupture of the membrane, individual development of faster individuals began to swim in the water, take the initiative to eat; 45d after rupture of membranes, yolk sac basically absorbed completely larvae swimming ability, Almost all go up.