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目的:研究HBV S基因亚型与前C基因1896位点变异的关系。方法:对104例病人血清用PCR技术特异性扩增前C基因1896位点突变和用PCR-RFLP技术对HBV S基因分型。结果:①1896位点变异检出率为63.46％;②武汉地区HBVS基因亚型Ⅰ感染率为68.27％,亚型Ⅱ为25.00％,亚型Ⅲ为4.81％,亚型Ⅰ+Ⅱ复合感染为1.92％;③在亚型Ⅱ中,单纯野生株感染为80.77％,单纯变异株为7.7％,野生株变异株同时感染为11.54％,而占感染多数的亚型Ⅰ中,野生株和变异株的感染无差异。结论:1896位点是高频率变异位点,其变异不是随机的,而是与HBV不同的S基因亚型有关,基因亚型Ⅱ不易发生1896位点变异,受感染的机体内环境只是起促进变异的作用。
Objective: To study the relationship between HBV S subtype and mutation of 1896 site of pre-C gene. Methods: PCR was used in 104 patients to amplify 1896 site mutation of pre-C gene and PCR-RFLP to genotype HBV S gene. Results: ① The detection rate of mutation at1896were 63.46%. ② The prevalence of HBV S genotypeⅠ in Wuhan was 68.27%, subtype Ⅱ was 25.00%, subtype Ⅲ was 4.81%, and subtype Ⅰ + Ⅱ was 1.92 %. In subtype Ⅱ, the infection rate of the wild-type strain was 80.77%, that of the pure-mutant strain was 7.7%, while that of the wild-type strain was 11.54% No difference in infection. CONCLUSION: The 1896 locus is a high-frequency mutation site whose mutation is not random but related to the different S gene subtypes of HBV. Genotype Ⅱ is not prone to 1896 locus mutation, and the infected environment in the organism only serves to promote The role of variation.