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目的 研究反义CⅡTA基因对心肌肌凝蛋白诱导的实验性自身免疫性心肌炎 (experi mentalautoimmunemyocarditis,EAM)的治疗作用。方法 以携带反义CⅡTA基因的腺病毒载体对肌凝蛋白免疫后的小鼠进行静脉注射 ,同时设立空载病毒组与单纯免疫组作为对照 ,以多种手段观察反义CⅡTA基因对EAM的治疗效果。结果 治疗组与两对照组相比 ,心肌病理损伤程度减轻 ;血浆抗心肌肌凝蛋白抗体滴度及cTnⅠ浓度明显下降 (P <0 .0 5 ) ,流式细胞术检测外周血与脾脏T细胞I Ad表达量明显下降 (P <0 .0 5 )。结论 反义CⅡTA基因对心肌肌凝蛋白诱导的EAM具有较好的治疗作用。
Objective To investigate the therapeutic effect of antisense C ¢ òTA on cardiac myosin-induced experimental autoimmune myocarditis (EAM). Methods The mice immunized with myosin were injected intravenously with the adenoviral vector carrying antisense CⅡTA gene. At the same time, the empty-virus group and the simple immunization group were set as the control, and the antisense CⅡTA gene therapy on EAM effect. Results Compared with the two control groups, the degree of myocardial pathological lesion was reduced in the treatment group. The antimyocardiomycin antibody titers and cTnⅠ concentrations were significantly decreased in the treatment group (P <0.05). The flow cytometry was used to detect T cells in peripheral blood and spleen I Ad expression decreased significantly (P <0.05). Conclusion Antisense C Ⅱ TA gene has a good therapeutic effect on cardiac myosin-induced EAM.