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目的了解辽宁地区副溶血弧菌腹泻病例分离株的表型及基因型特征。方法对2012—2013年辽宁地区101株副溶血弧菌临床分离株进行血清型分型;采用微量肉汤法,进行16种抗菌药物的药敏试验;通过PCR方法对其进行tlh、tdh和trh毒力基因检测;采用脉冲场凝胶电泳(PFGE)方法对菌株进行基因分型。结果101株副溶血弧菌分属于25种血清型,其中O3:K6为优势血清型,占37.62%(38/101);对磺胺甲恶唑、链霉素、氨苄西林产生耐药;对环丙沙星、强力霉素、头孢吡肟、甲氧苄啶、亚胺培南、头孢曲松钠等均具有较高敏感性;全部菌株tlh基因阳性;tdh存在于大部分菌株中,所占比例为84.16%(85/101);全部菌株trh基因阴性;O3:K6型菌株tdh基因阳性率100%,明显高于非O3:K6型菌株(76.19%,77/101)(P=0.000 81)。101株副溶血弧菌分成63种型别,38株O3:K6型菌株分成19种型别,带型分散,无明显聚集性。结论辽宁省感染性腹泻病例副溶血弧菌分离株以O3:K6型为主;毒力基因tlh和tdh携带率高,且O3:K6型临床分离株毒力更强;副溶血弧菌对多数抗生素均具有较高敏感性;PFGE结果提示辽宁地区流行的副溶血弧菌存在多克隆来源。
Objective To understand the phenotypes and genotypes of isolates of Vibrio parahaemolyticus diarrhea in Liaoning Province. Methods The clinical isolates of 101 strains of Vibrio parahaemolyticus in Liaoning Province from 2012 to 2013 were serotyped. The antibiotic susceptibility of 16 antibiotics was tested by micro broth method. The tlh, tdh and trh Virulence genes were detected. The strains were genotyped by pulsed-field gel electrophoresis (PFGE). Results 101 Vibrio parahaemolyticus belonged to 25 serotypes, of which O3: K6 was the predominant serotype, accounting for 37.62% (38/101), resistant to sulfamethoxazole, streptomycin and ampicillin, Ciprofloxacin, doxycycline, cefepime, trimethoprim, imipenem, ceftriaxone, etc. all have high sensitivity; all strains tlh gene positive; tdh exist in most strains, accounting for The positive rate of tdh gene was 100% in O3: K6 strain, which was significantly higher than that in non-O3: K6 strain (76.19%, 77/101) (P = 0.000 81 ). 101 strains of Vibrio parahaemolyticus were divided into 63 types, 38 strains of O3: K6 strains were divided into 19 types, which were scattered with no obvious aggregation. Conclusion The isolates of Vibrio parahaemolyticus in infectious diarrhea in Liaoning Province are mainly O3: K6 type. The virulence genes tlh and tdh are highly virulent and the strains of O3: K6 strains are more virulent. Antibiotics are highly sensitive; PFGE results suggest that there are polyclonal sources of Vibrio parahaemolyticus in Liaoning.