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目的研究矢车菊黄素(centaureidin)在Caco-2细胞单层模型中的吸收机制。方法以HPLC分析矢车菊黄素浓度,用Caco-2细胞单层模型评价吸收时间、药物浓度、介质pH值、抑制剂等对矢车菊黄素吸收的影响,研究矢车菊黄素的吸收机制,计算表观渗透系数(apparent permeability coefficient,Papp)。结果药物的吸收与药物浓度和吸收时间正相关;弱酸性介质条件下有利于药物的吸收;2,4-二硝基酚(DNP)对药物吸收无影响,但异博定(verapamil)可增加药物的吸收;从肠腔侧到基底侧的转运小于基底侧到肠腔侧的转运。结论矢车菊黄素在Caco-2细胞模型中的吸收主要是被动转运,受P-糖蛋白的外排作用。
Aim To investigate the mechanism of absorption of centaureidin in monolayers of Caco-2 cells. Methods The concentration of cyanidin in cartilage was determined by HPLC. The effect of absorption time, drug concentration, pH value and inhibitor on the absorption of flavin in cornflower was evaluated by Caco-2 monolayer model. Absorption mechanism to calculate apparent permeability coefficient (Papp). Results The absorption of drug was positively correlated with drug concentration and absorption time; the absorption of drug was favored under mild acidic medium; 2,4-dinitrophenol (DNP) had no effect on drug absorption, but verapamil increased Drug absorption; transport from the luminal side to the basal side is less than that from the basal side to the luminal side. Conclusion The absorption of cyanidin in Caco-2 cell model is mainly passive and is mediated by the efflux of P-glycoprotein.