论文部分内容阅读
目的探讨宫颈良恶性病变组织中EZH2和PTEN蛋白的表达水平及其临床意义。方法采用免疫组织化学SP法检测正常宫颈组织30例(对照组)、宫颈上皮内瘤变组织78例(CIN组)和宫颈鳞癌组织78例(宫颈鳞癌组)中EZH2和PTEN的表达情况。结果宫颈鳞癌组EZH2蛋白表达阳性率明显高于CIN组与对照组,PTEN蛋白表达阳性率明显低于CIN组与对照组(P<0.01);宫颈鳞癌中EZH2与PTEN蛋白表达在组织学分期、淋巴转移及浸润深度上差异有统计学意义(P<0.01),在年龄、临床分期上差异无统计学意义(P>0.05);EZH2的表达与PTEN呈负相关(r=-0.675,P<0.05)。结论 EZH2和PTEN蛋白可能在宫颈鳞癌发生、发展过程中起重要作用,可作为判定宫颈鳞癌恶性程度及进程的重要参考指标。
Objective To investigate the expression of EZH2 and PTEN protein in benign and malignant cervical lesions and their clinical significance. Methods Immunohistochemical SP method was used to detect the expression of EZH2 and PTEN in 30 cases of normal cervical tissue (control group), 78 cases of cervical intraepithelial neoplasia (CIN group) and 78 cases of cervical squamous cell carcinoma (cervical squamous cell carcinoma group) . Results The positive rate of EZH2 protein in cervical squamous cell carcinoma was significantly higher than that in CIN and control groups (P <0.01). The positive rates of PTEN protein in cervical squamous cell carcinoma were significantly lower than those in CIN and control groups (P <0.01) There was no significant difference in age, clinical stage (P> 0.05). The expression of EZH2 was negatively correlated with PTEN (r = -0.675, P <0.05). Conclusions EZH2 and PTEN proteins may play an important role in the occurrence and development of cervical squamous cell carcinoma, which may be used as an important reference index for determining the malignancy and progression of cervical squamous cell carcinoma.