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目的:采用响应面法优化红豆杉枝叶紫杉烷类萃余物(TREFT)中黄酮类物质的超临界流体萃取工艺。方法:根据Central Composite Design设计原理,采用四因素五水平的响应面分析法,分别考察萃取时间、萃取温度、萃取压力和夹带剂乙醇浓度对紫杉黄酮及单体组分阿曼托黄酮、槲皮素、银杏素提取率的影响。结果:优化工艺条件为:萃取时间2.2 h,萃取温度46.4℃,萃取压力22.6 MPa,夹带剂乙醇浓度80.7%,紫杉黄酮及单体组分提取率的综合评分为98.75。结论:优选的超临界流体萃取紫杉黄酮及单体组分工艺稳定可行,可为TREFT资源化利用提供技术支持。
OBJECTIVE: To optimize the supercritical fluid extraction process of flavonoids in Taxus ramie leaves (TREFT) by response surface methodology. Methods: According to the design principle of Central Composite Design, four factors and five levels of response surface analysis were used to investigate the effects of extraction time, extraction temperature, extraction pressure and entrainer ethanol concentration on the bioactivity of the components, Su, the extraction rate of ginkgolides. Results: The optimal extraction conditions were as follows: extraction time 2.2 h, extraction temperature 46.4 ℃, extraction pressure 22.6 MPa, entrainer ethanol concentration 80.7%, and total yield of taxoids and monomer components was 98.75. CONCLUSION: The technology of extracting supernatant from taxanes and monomer components by supercritical fluid is stable and feasible, which can provide technical support for the utilization of TREFT resources.