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目的 研究老年人胃癌和直肠癌中的 C- MYC基因扩增情况。方法 用聚合酶链式反应 (PCR)测定 84例老年胃癌和直肠癌患者中的 C- MYC基因 ,通过激光密度扫描仪对琼脂糖凝胶 EB染色底片进行积分光密度分析。结果 正常组织无 C- MYC扩增 ,C-MYC/N+L- MYC比值 95%可信区间为 0 .0 86 9～ 0 .62 5 7,胃癌组织 C- MYC基因扩增阳性率为 69.2 % ,直肠癌组织的 C- MYC基因扩增阳性率为 62 .5% ,84例肿瘤组织 C- MYC扩增总阳性率为 66.7%。结论 癌组织 C- MYC扩增高于正常组织 ,在不同肿瘤组织中扩增程度不同 ,与细胞潜在的增殖能力成正相关 ;这可为肿瘤的发生、发展及预后 ,特别为指导基因治疗提供有用的资料。
Objective To study the amplification of C-MYC gene in elderly patients with gastric cancer and rectal cancer. Methods The C-MYC gene in 84 elderly patients with gastric cancer and rectal cancer was determined by polymerase chain reaction (PCR). The integrated optical densitometry of agarose gel EB negative was analyzed by laser density scanner. Results There was no amplification of C-MYC in normal tissues. The 95% confidence interval of C-MYC/N+L-MYC ratio was 0. 0 86 9 to 0. 62 5 7. The positive rate of C-MYC gene amplification was 69.2 in gastric cancer tissues. The positive rate of C-MYC gene amplification in rectal cancer tissue was 62.5%, and the total positive rate of C-MYC in 84 tumor tissues was 66.7%. Conclusions The C-MYC amplification in cancer tissue is higher than that in normal tissues, and the degree of amplification in different tumor tissues is different. It is positively correlated with the cell’s potential proliferative capacity. This may be the occurrence, development and prognosis of tumors, and is particularly useful for guiding gene therapy. data of.