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目的 :探讨超声引导下细针穿刺与基因检测相结合早期诊断胰腺癌的临床应用。方法 :应用超声引导下细针穿刺技术对 38例可疑胰腺癌及其邻近器官占位性病变患者进行穿刺活检 ,同时采用聚合酶链反应—限制性酶切片段长度多态性技术 ,对所取得的标本 c- ki- ras基因第 12密码子突变进行检测 ,应用于胰腺癌的诊断。结果 :2 2例胰腺癌中有 2 1例有 c- ki- ras基因第 12密码子突变 ,多为 GGT变成 GAT、 GCT、 GTT,阳性率达 95 .4% ;而 16例慢性胰腺炎 ,壶腹癌、胰岛素瘤等均无 c- ki- ras基因第 12密码子突变。结论 :将超声引导下细针穿刺与胰腺癌 c-ki- ras基因第 12密码子突变检测相结合是诊断和鉴别诊断胰腺癌的有效方法
Objective : To explore the clinical application of ultrasound-guided fine needle aspiration and gene detection in the early diagnosis of pancreatic cancer. METHODS: Ultrasound-guided fine-needle aspiration biopsy was performed on 38 cases of suspected pancreatic cancer and adjacent organ space-occupying lesions. Biopsy was performed using polymerase chain reaction-restriction fragment length polymorphism. The mutation of the 12th codon of the c-kiras gene in the specimen was examined for the diagnosis of pancreatic cancer. RESULTS: Of the 22 cases of pancreatic cancer, 21 had a mutation in codon 12 of the c-kiras gene. Most of them were GGT, GAT, GCT, and GTT. The positive rate was 95.4%; 16 cases of chronic pancreatitis. There was no mutation at codon 12 of the c-kiras gene in ampullary carcinoma and insulinoma. Conclusion: The combination of ultrasound-guided fine needle aspiration and mutation detection of codon 12 of co-ki-ras gene in pancreatic cancer is an effective method for the diagnosis and differential diagnosis of pancreatic cancer.