论文部分内容阅读
目的：观察降钙素基因相关肽（ＣＧＲＰ）对阿霉素所致心肌细胞毒性作用的影响．方法：采用原代培养乳鼠心肌细胞，以１０－６ｍｏｌ／Ｌ阿霉素造成急性心肌细胞毒性模型，ＣＧＲＰ作用浓度为１０－８ｍｏｌ／Ｌ．测定培养基中乳酸脱氢酶（ＬＤＨ）活性及心肌细胞内丙二醛（ＭＤＡ）、钙和镁含量．结果：阿霉素引起心肌细胞ＬＤＨ漏出明显增加，细胞内ＭＤＡ，钙含量水平明显升高，镁含量水平明显降低．阿霉素所致心肌细胞ＬＤＨ漏出与细胞内ＭＤＡ含量水平呈正相关．ＣＧＲＰ可明显减轻阿霉素引起的心肌细胞ＬＤＨ漏出及细胞内ＭＤＡ，钙的蓄积和镁的丢失．结论：ＣＧＲＰ通过抑制脂质过氧化反应和减轻细胞内钙超载对阿霉素心肌细胞毒性具有保护作用．
Objective: To observe the effect of calcitonin gene related peptide (CGRP) on doxorubicin-induced cardiomyocyte cytotoxicity. Methods: Primary cultured neonatal rat cardiomyocytes were treated with 10-6 mol / L doxorubicin to induce acute myocardial cytotoxicity. The concentration of CGRP was 10-8 mol / L. The activity of lactate dehydrogenase (LDH) and the contents of malondialdehyde (MDA), calcium and magnesium in myocardial cells were determined. Results: Doxorubicin caused a significant increase of LDH leakage in cardiomyocytes. The levels of MDA and Ca2 + in the cells were significantly increased, while the levels of Mg in the cells were significantly decreased. Doxorubicin induced cardiomyocyte LDH leakage and intracellular levels of MDA was positively correlated. CGRP can significantly reduce doxorubicin-induced myocardial LDH leakage and intracellular MDA, calcium accumulation and magnesium loss. CONCLUSION: CGRP has a protective effect on doxorubicin cytotoxicity by inhibiting lipid peroxidation and reducing intracellular calcium overload.