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为探讨天蚕素和溶菌酶对中华鳖Trionyx sinensis腐皮病的防治效果。本实验采用肌肉注射天蚕素(10 mg·kg~(-1))、溶菌酶(10 mg·kg~(-1))及天蚕素(6 mg·kg~(-1))和溶菌酶(5 mg·kg~(-1))混合物,观察其对鳗气单胞菌Aeromonas encheleia引起的中华鳖腐皮病的预防效果。以死亡率和相对保护率评价不同药物的保护效果;通过检测血清凝集效价、细胞吞噬功能和血清杀菌活力,比较不同药物对中华鳖免疫力和抗病能力的影响;比较庆大霉素和溶菌酶血药残留浓度,评估药物毒副作用。结果表明:天蚕素和溶菌酶二联法对中华鳖保护效果最好、免疫效果最佳,与其他组相比差异均有高度统计学意义(P<0.01);血药残留分析表明,溶菌酶在用药后第6天全部代谢完毕,庆大霉素停药第12天仍有残留。天蚕素和溶菌酶二联法预防中华鳖腐皮病效果显著优于抗生素,无药物残留和耐药副作用,并提高免疫力,避免了应用抗生素所带来的危害,值得推广应用。
To investigate the effect of Cecropin and Lysozyme on the control of Trionyx sinensis skin disease. In this study, intramuscular injection of cecropin (10 mg · kg -1), lysozyme (10 mg · kg -1) and cecropin (6 mg · kg -1) and lysozyme 5 mg · kg ~ (-1)) mixture was used to observe the preventive effect of Aeromonas encheleia on the skin of Chinese soft-shelled turtle. The protective effect of different drugs was evaluated by mortality and relative protection rate. The effects of different drugs on immunity and disease resistance of soft-shelled turtle were compared by detecting serum agglutination titer, phagocytic function and serum bactericidal activity. The effects of gentamicin and Lysozyme blood drug residual concentration, evaluation of drug side effects. The results showed that the combination of Cecropin and Lysozyme had the best protective effect on Trionyx sinensis and the best immunization effect compared with other groups (P <0.01). The analysis of plasma drug residues showed that lysozyme After 6 days after treatment, all metabolism was completed, there was still residue on the 12th day of gentamicin withdrawal. The combination of cecropin and lysozyme in the prevention of rot disease of Trionyx sinensis is significantly better than antibiotics, no drug residues and drug side effects, and improve immunity, to avoid the harm caused by the application of antibiotics, it is worth to promote the application.