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对采集于农业堆肥高温期的微生物样品进行30d的高温驯化,驯化完成后从中筛选得到15株嗜热纤维素分解菌,经形态学特征、生理生化反应及16S rDNA序列同源性比对鉴定为:Bacillus sp3株,Paenibacillus sp1株,Bacillus licheniformis3株,Bacillus subtilis4株,Brevibacillus borstelensis1株,Bacillus coagulans3株.将筛选得到的菌种添加至高纤维素含量的堆体进行效果验证,结果表明添加嗜热纤维素分解菌对堆肥pH变化无显著影响,但可提高堆肥高温期温度、延长高温期并显著地降低堆肥产品的C/N和有机质含量,显著降低纤维素和半纤维素含量,加快堆肥腐熟.堆体有机质降解动力学结果表明,堆体接菌和不接菌处理的有机质最大降解度分别为62.5481%和61.7101%,速率常数分别为0.1250d-1和0.1051d-1,接菌处理的堆肥比对照堆肥提前6d达到稳定.研究表明,添加筛选的嗜热纤维素分解菌能缩短堆肥周期.
Fifteen strains of thermophilic cellulolytic bacteria were screened out from the microbial samples collected during the high temperature stage of agricultural composting for 30 days. After morphological, physiological and biochemical reactions and 16S rDNA sequence homology comparison, they were identified as : Bacillus sp3, Paenibacillus sp1, Bacillus licheniformis 3, Bacillus subtilis 4, Brevibacillus borstelensis 1, Bacillus coagulans 3. The selected strains were added to the high cellulose content of the pile for validation, the results show that the addition of thermophilic cellulose Decomposing bacteria had no significant effect on the pH of composting, but could increase the temperature of composting at high temperature, extend the high temperature period and significantly reduce the C / N and organic matter content of compost products, significantly reduce the content of cellulose and hemicellulose, and accelerate the compost maturity. The degradation kinetics of somatic organic matter showed that the maximal degradation rates of organic matter by both inoculation and non-inoculation were 62.5481% and 61.7101%, respectively. The rate constants were 0.1250d-1 and 0.1051d-1, respectively. The control compost stabilized 6 days earlier.Research shows that the addition of screening thermophilic cellulolytic bacteria can shorten the compost cycle.