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目的 建立和评价改变血脑屏障通透性的动物模型。方法 利用动物低压舱,通过5000m急性低压缺氧暴露5h后立即心脏灌注硝酸镧固定液,制成大鼠血脑屏障通透性改变模型,并置透射电镜下观察其组织形态学变化。结果 实验组形态学表现为镧颗粒通过大鼠脑皮质的毛细血管内皮细胞膜,沉积于毛细血管内皮细胞浆内、细胞核膜上以及血管周围,弥漫性地分布于脑间质细胞间隙,并附着于神经细胞膜上。对照组镧颗粒仅滞留于大脑皮质的毛细血管腔内。结论 用急性低压缺氧暴露制成血脑屏障通透性改变的动物模型方法简便,成功率高。
Objective To establish and evaluate an animal model for changing the permeability of blood-brain barrier. Methods Animal hypobaric chambers were used to establish the model of permeability changes of blood-brain barrier in rats after acute hypobaric hypoxia exposure at 5000m for 5h, and the morphological changes of blood-brain barrier were observed under transmission electron microscope. Results The morphology of the experimental group was that lanthanum particles passed through the capillary endothelial cell membrane of the rat cerebral cortex and deposited on the cytoplasm of the capillary endothelial cell, the nuclear membrane and the perivascular area diffusely distributed in the interstitial space of the brain stroma and attached to Nerve cell membrane. Lanthanum particles in the control group only remained in the capillary lumen of the cerebral cortex. Conclusion The acute hypobaric hypoxic exposure to the blood-brain barrier permeability changes in animal models is simple and high success rate.