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Lithium-sulfur (Li-S) battery with a new configuration is demonstrated by inserting a flexible nitrogen-doping carbon nanofiber (N-CNFs) interlayer between the sulfur cathode and the separator.The N-CNFs film with high surface roughness and surface area is fabricated by electrospinning and a subsequent calcination process.The N-CNFs film interlayer not only effectively traps the shuttling migration of polysulfides but also gives the whole battery reliable electronic conductivity,which can effectively enhance the electrochemical performance of Li-S batteries.Finally,Li-S batteries with long cycling stability of 785 mAh/g after 200 cycles and good rate capability of 573 mAh/g at 5 C are achieved.