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目的比较哮喘患者与健康受试者尿液中水通道蛋白2(aquaporin 2,AQP2)的含量,探讨哮喘不同临床控制水平AQP2含量的差异及与炎症介质的相关性。方法检测60例哮喘患者及21例健康受试者(正常对照组)尿液中AQP2含量;CBA技术检测血浆白介素(IL)-4、IL-6、肿瘤坏死因子(TNF)-α及干扰素(IFN)-γ水平。结果哮喘未控制组(ACT在19分以下)及部分控制组(ACT 20~24分)患者尿液AQP2水平均较正常对照组增高(P<0.01),且未控制组增高明显,达(174.28±40.81)pg/mL;血浆IL-4含量在哮喘未控制组最高(1.10±0.25)pg/mL,与正常对照组及哮喘部分控制组比较,差异有统计学意义(P<0.01);血浆IL-6含量在哮喘未控制组最高,但与正常对照组及哮喘部分控制组比较,差异无统计学意义(P=0.058);TNF-α含量哮喘组高于对照组,差异有统计学意义(P<0.05)。尿液AQP2与血浆IL-4、IL-6均有相关性(P=0.049,P=0.010),IL-4与IFN-γ相关(P=0.019),IL-6与TNF-α有相关性(P=0.010);IL-10与IFN-γ相关(P=0.005)。结论哮喘患者尿液AQP2水平增高,且与血浆炎症介质IL-4、IL-6相关,尿液AQP2在支气管哮喘发病机制中的作用值得进一步研究。
Objective To compare the urinary levels of aquaporin 2 (AQP2) in patients with asthma and healthy subjects and to explore the differences of the levels of AQP2 in different clinically controlled levels of asthma and its correlation with inflammatory mediators. Methods Urinary levels of AQP2 were measured in 60 asthmatic patients and 21 healthy controls (normal control group). Plasma levels of IL-4, IL-6, TNF-α and interferon (IFN) -γ level. Results Urine AQP2 levels in asthma uncontrolled group (ACT lower than 19) and partial control group (ACT 20-24) were significantly higher than those in normal control group (P <0.01), and were significantly higher in uncontrolled group (174.28 ± 40.81) pg / mL. The plasma IL-4 level was the highest (1.10 ± 0.25) pg / mL in the uncontrolled asthma group, which was significantly different from the normal control group and asthma control group (P <0.01) The content of IL-6 was the highest in uncontrolled asthma group, but there was no significant difference between normal control group and asthma control group (P = 0.058); TNF-αlevel in asthma group was higher than that in control group, the difference was statistically significant (P <0.05). AQP2 in urine was correlated with plasma IL-4 and IL-6 (P = 0.049, P = 0.010), IL-4 was correlated with IFN-γ (P = 0.019) (P = 0.010); IL-10 was associated with IFN-γ (P = 0.005). Conclusions The level of AQP2 in urine of asthmatic patients is increased, and it is correlated with IL-4 and IL-6 in plasma inflammatory mediators. The role of urine AQP2 in the pathogenesis of bronchial asthma deserves further study.