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碱性介质中K3Fe(CN)6与H2-受体拮抗剂发生氧化-还原反应产生化学发光,罗丹明6G可显著增强其发光信号,且强度与药物浓度呈良好的线性关系。据此,建立了采用K3Fe(CN)6-罗丹明6G体系测定制剂中H2-受体拮抗剂的流动注射-化学发光分析方法。在优化的实验条件下,西咪替丁在1.0×10-9~7.0×10-5g/mL范围内呈良好的线性关系,检出限为8.6×10-10g/mL;盐酸雷尼替丁在1.0×10-9~5.0×10-5g/mL范围内呈线性关系,检出限为8.7×10-10g/mL;法莫替丁在5.0×10-9~7.0×10-5g/mL范围内呈线性关系,检出限为2.4×10-9g/mL。通过对浓度为1.0×10-6g/mL的3种H2-受体拮抗剂分别进行的11次平行测定,其RSD分别为4.0%,3.8%和2.9%。
The redox reaction of K3Fe (CN) 6 with H2-receptor antagonist in alkaline medium produced chemiluminescence. Rhodamine 6G could significantly enhance the luminescence signal, and the intensity showed a good linear relationship with the drug concentration. Based on this, a flow-injection chemiluminescence method for the determination of H2-receptor antagonists in K3Fe (CN) 6-rhodamine 6G system was established. Under the optimized experimental conditions, the cimetidine showed a good linear relationship in the range of 1.0 × 10-9 ~ 7.0 × 10-5g / mL with the detection limit of 8.6 × 10-10g / mL. Ranitidine hydrochloride The linear range was 1.0 × 10-9 ~ 5.0 × 10-5g / mL. The limit of detection was 8.7 × 10-10g / mL. Famotidine was detected in 5.0 × 10-9 ~ 7.0 × 10-5g / mL The range of linear relationship, the detection limit of 2.4 × 10-9g / mL. The RSDs were 4.0%, 3.8% and 2.9%, respectively, by 11 parallel determinations of three H2-receptor antagonists at a concentration of 1.0 × 10-6 g / mL, respectively.