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目的:探讨三七总皂苷(total saponins of Panax notoginseng,PNS)对大鼠睾丸曲细精管精子发生的影响。方法:20只雄性SD大鼠随机均分为对照组与3个实验组,每组5只动物。实验组经口灌胃分别给予含PNS300mg/kg(B组)、600mg/kg(C组)、900mg/kg(D组)的生理盐水1ml,对照组(A组)以等量生理盐水代替,连续给药14d。采用HE染色法观察睾丸曲细精管生精上皮和间质区的组织学改变,比较4组的睾丸每日精子生成量(DSP)和异常曲细精管率。结果:B和C组的睾丸组织学与A组的相似,D组的可见病理改变明显的曲细精管。3个实验组的间质区未见异常改变。3个实验组中以D组的DSP最低及异常曲细精管率最高,但3组的DSP和异常曲细精管率分别与A组的比较无统计学差异(P>0.05)。结论:本实验在组织学水平未显示PNS引起睾丸精子发生有显著的变化,但高剂量长期给予PNS对曲细精管的影响需予以重视。
Objective: To investigate the effects of total saponins of Panax notoginseng (PNS) on spermatogenesis of testicular seminiferous tubules in rats. Methods: Twenty male Sprague-Dawley rats were randomly divided into control group and three experimental groups, with 5 animals in each group. The experimental group was orally administrated with 1 ml normal saline containing PNS 300 mg/kg (group B), 600 mg/kg (group C), and 900 mg/kg (group D), and the control group (group A) was replaced with the same amount of physiological saline. Continuous administration 14d. HE staining was used to observe the histological changes in the spermatogenic epithelium and interstitium of the testicular seminiferous tubules. The daily sperm production (DSP) and abnormal seminiferous tubule ratio of the testicles were compared. Results: Testicular histology in group B and C was similar to that in group A. Visible pathological changes were observed in group D with seminiferous tubules. There was no abnormal change in the interstitial area of the three experimental groups. The lowest and abnormal seminiferous seminiferous tubules were the highest in DSP in the three experimental groups, but there was no significant difference between the DSP and the abnormal seminiferous tubule ratios in Group A and Group A (P>0.05). Conclusion: This experiment does not show that PNS causes significant changes in testicular spermatogenesis at the histological level, but the effect of high dose long-term administration of PNS on seminiferous tubules needs attention.