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在添加2.0mg/L6-BA和0.5mg/LNAA的MS培养基上,通过14d的诱导,茎可获得100%的诱导率。叶片愈伤组织在添加l.5mg/L6-BA和1.0mg/LNAA的MS培养基上诱导16d,可获得100%的诱导率。用分光光度法直接筛选出高类黄酮含量的红色系,其类黄酮含量为5.7855mg/g,是含量最低的白色系的2.7倍。获得的高类黄酮含量的愈伤系为进一步的细胞系悬浮培养和药用次生代谢产物的规模化生产奠定了基础;同时,也为研究荞麦类黄酮次生代谢的分子调控提供了优异的材料。
On the MS medium supplemented with 2.0 mg / L 6-BA and 0.5 mg / L NAA, the induction rate of 100% was obtained by the stem for 14 days. Leaf callus induction on MS medium supplemented with 1.5 mg / L 6-BA and 1.0 mg / L NAA for 16 days resulted in 100% induction. The red line with high flavonoid content was screened directly by spectrophotometry. The flavonoid content was 5.7855mg / g, which was 2.7 times of the white line with the lowest content. The obtained callus with high flavonoid content laid the foundation for further cell suspension culture and large-scale production of medicinal secondary metabolites. At the same time, it also provided an excellent method for studying the molecular regulation of buckwheat flavonoid secondary metabolism material.