由玉米获白×莱1029的幼胚诱导愈伤组织,经改造后进行液体悬浮培养,获得了优良的、有再生能力的悬浮细胞系。用其分离原生质体,采用4种方式进行培养,最高原生质体植板率达到1.2％。原生质体再生的小愈伤组织,通过分步诱导分化法,获得了5株完整的绿色植株,经进一步的壮苗培养后,移栽到土壤中全部成活,最终雄穗结籽。后代植株育性正常。 The maize was white × Lai 1029 immature embryos induced callus, after the transformation of the liquid suspension culture, access to an excellent, reproducible suspension cell line. Protoplasts were isolated using these four methods and the highest protoplast plating rate reached 1.2%. Small callus regenerated from protoplasts obtained five complete green plants by stepwise induction and differentiation method. After further seedling culture, all the callus were transplanted into the soil and survived successfully. Offspring plant fertility normal.