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探讨单用硫唑嘌呤及与甲基强的松龙合用，对慢性脑血管痉挛的防治作用。方法采用成年家犬，于实验第１天和第４天分别在枕大池穿刺注血（０．３ｍｌ／ｋｇ），建立蛛网膜下腔出血模型，观察免疫抑制剂硫唑嘌呤单用和与甲基强的松龙合用对脑血管痉挛的预防作用。并进行椎动脉造影、管壁自由基测定、管壁组织学检查等观察其疗效。结果在蛛网膜下腔出血后第７天，硫唑嘌呤组的基底动脉口径为８７％±２６％，管壁脂质过氧化物（ＬＰＯ）含量为０．０３±０．０１ｎｍｏｌ／Ｌ；硫唑嘌呤＋甲基强的松龙组分别为９３％±２０％和０．０２±０．０１ｎｍｏｌ／Ｌ；而对照组为５３％±１９％，ＬＰＯ含量为０．１１±０．０５ｎｍｏｌ／Ｌ。组织学检查发现硫唑嘌呤组及硫唑嘌呤＋甲基强的松龙组的血管壁结构破坏明显，较对照组轻。结论免疫抑制剂能够预防蛛网膜下腔出血后的慢性脑血管痉挛，减少自由基的产生，减轻血管壁的损害；联合应用比单用药量少，效果大。
To explore the combination of azathioprine and methylprednisolone on the prevention and treatment of chronic cerebral vasospasm. Methods Adult puppies were randomly divided into three groups: one was on the first day and the other was on the fourth day. The model of subarachnoid hemorrhage was established by puncture and injection (0.3ml / kg) on the occipital cistern. The immunosuppressive agents azathioprine alone and Preventive effect of combination of prednisolone on cerebral vasospasm. Vertebral artery angiography, wall free radical assay, histological examination of the wall were performed to observe the curative effect. Results On the 7th day after subarachnoid hemorrhage, the diameter of basilar artery of azathioprine group was 87% ± 26% and the content of LPO was 0.03 ± 0.01nmol / L. The sulfur The levels of azathioprine and methylprednisolone were 93% ± 20% and 0.02 ± 0.01nmol / L, while the control group was 53% ± 19% and the LPO content was 0.11 ± 0.05nmol / L . Histological examination found that the azathioprine group and azathioprine + methyl prednisolone group of vascular wall damage was significantly lighter than the control group. Conclusion Immunosuppressive agents can prevent chronic cerebral vasospasm after subarachnoid hemorrhage, reduce the production of free radicals and reduce the damage of the vascular wall. The combined application is less effective than single administration and has great effect.