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目的探讨经动脉灌注化疗对乳腺癌雌激素(estrogen receptor,ER)和孕激素受体(progesterone receptor,PR)表达的影响。方法应用逆转录聚合酶链式反应(RT-PCR)和免疫组化方法检测81例乳腺癌患者经动脉灌注化疗前后的雌孕激素受体表达情况。采用Western blot检测经动脉灌注化疗前后ER和PR的变化。结果RT-PCR结果表明,81例患者中43例(53.1%)ERmRNA水平升高,33例(40.7%)PRmRNA水平升高,25例(30.9%)ER和PRmRNA水平同时升高。免疫组织化学结果提示,35例(43.2%)ER表达水平升高,其中14例(17.3%)由阴性转为阳性;21例(26.0%)PR表达水平升高,其中10例(12.2%)由阴性转为阳性;16例(19.8%)ER和PR表达同时升高。Wester nblot显示,经动脉灌注化疗后,81例患者中38例(46.9%)ER蛋白水平升高,30例(37.0%)PR蛋白水平升高,21例(25.9%)ER和PR蛋白水平同时升高。结论经动脉灌注化疗可有效上调ER、PRmRNA和蛋白水平的表达,有利于后续化疗药物对肿瘤细胞的杀灭。
Objective To investigate the effects of intra-arterial chemotherapy on the expression of estrogen receptor (ER) and progesterone receptor (PR) in breast cancer. Methods The expression of estrogen and progesterone receptors in 81 breast cancer patients before and after arterial infusion chemotherapy was detected by RT-PCR and immunohistochemistry. Western blot was used to detect the changes of ER and PR before and after arterial infusion chemotherapy. Results The results of RT-PCR showed that the ER mRNA level increased in 43 cases (53.1%), the PR mRNA level in 33 cases (40.7%) increased, and the ER and PR mRNA levels increased in 25 cases (30.9%). The results of immunohistochemistry showed that ER expression increased in 35 cases (43.2%), of which 14 cases (17.3%) changed from negative to positive. The expression of PR in 21 cases (26.0%) increased, of which 10 cases (12.2% Negative to positive; 16 cases (19.8%) ER and PR expression increased at the same time. Westerblot showed that 38 (46.9%) of the 81 patients had elevated ER protein levels, 30 (37.0%) of the elevated PR protein levels, and 21 (25.9%) of the ER and PR protein levels simultaneously after arterial infusion chemotherapy Rise. Conclusion Intra-arterial infusion chemotherapy can effectively up-regulate the expression of ER, PR mRNA and protein, which is beneficial to the subsequent killing of tumor cells by chemotherapeutics.