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以山葡萄品种中抗性较强的‘左山一’和抗性较弱的‘双丰’品种为材料,利用气体交换分析和叶绿素荧光诱导动力学分析手段,研究了干旱胁迫对不同抗性山葡萄品种的光合作用和PSII活性的影响。结果显示,2种山葡萄叶片的净光合速率均随着干旱胁迫的加重而下降,并且细胞间隙CO2浓度升高,证明非气孔限制是干旱胁迫造成山葡萄光合速率下降的主要原因。JIP-test分析发现,干旱胁迫导致‘双丰’叶片相对荧光诱导动力学曲线中J点和I点相对可变荧光上升,同时出现了明显的K点,‘左山一’叶片的相对荧光诱导动力学曲线没有明显变化,说明干旱胁迫对‘双丰’叶片PSII电子供体侧和受体侧造成的伤害要显著大于对‘左山一’叶片的伤害,且干旱胁迫对‘双丰’叶片的最大光化学效率(Fv/Fm)、吸收光能为基础的光化学性能指数(PIABS)、单位面积有活性反应中心的密度(RS/CS)及单位面积用于电子传递的光能(ETo/CS)的改变幅度显著大于‘左山一’。干旱胁迫通过干扰山葡萄叶片PSII电子供体侧、受体侧以及电子传递链的功能,严重的伤害了叶片光合机构的正常功能。干旱胁迫对抗旱性较强的‘左山一’PSII活性的影响显著小于对抗旱性较弱的‘双丰’葡萄。
In this study, gas exchange analysis and chlorophyll fluorescence-induced kinetic analysis were used to investigate the effects of drought stress on different resistant grape varieties, such as ’Zushan I’ and ’Shuangfeng’ Varieties of photosynthesis and PSII activity. The results showed that the net photosynthetic rate of two grape leaves decreased with the increase of drought stress and the intercellular CO2 concentration increased. It was proved that the non-stomatal limitation was the main reason for the decrease of photosynthetic rate of grape under drought stress. JIP-test analysis showed that drought stress led to the relative variable fluorescence increase of J and I in the relative fluorescence induction kinetic curve of ’Double Fung’ leaves, and at the same time there was obvious K-point. The relative fluorescence induction of ’Zoshanyi’ There was no significant change in the kinetic curve, indicating that the damage caused by drought stress on donor and acceptor sides of PSII was significantly greater than that of ’Zanshanyi’ leaves, The maximum photochemical efficiency (Fv / Fm), the absorbed light energy based photochemical performance index (PIABS), the density of reactive centers per unit area (RS / CS) and the energy per unit area for electron transfer ) Was significantly larger than that of ’Zuo Shan Yi’. Drought stress seriously damaged the normal function of photosynthetic apparatus of leaves by interfering with the function of donor side, acceptor side and electron transfer chain of PSII electron of grape leaves. Drought stress had less effect on PSII activity of Zanshaniligou than that of drought resistant ones.