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目的建立高效毛细管电泳法(HPCE)同时测定乌头类药材中乌头碱、次乌头碱、新乌头碱、苯甲酰乌头原碱、苯甲酰次乌头原碱和苯甲酰新乌头原碱含量的方法。方法以40 mmol·L-1乙酸钠-60 mmol·L-1乙酸铵-80 mmol·L-1tris试剂-25%甲醇(pH 8.3)为缓冲溶液,未涂渍标准熔融石英毛细管(75μm×64.5 cm,有效长度56 cm)为分离通道,分离电压为16 kV,检测波长为235 nm,毛细管温度为25℃,压力进样为5 kPa×6 s。结果 6种生物碱的浓度与峰面积的线性关系良好(r>0.999 7);加样回收率为95.25%~103.91%。用此法测定了乌头类药材中6种生物碱的含量,得到满意结果。结论该方法简单、准确,重复性较好,可用于乌头类药材内在质量的评价和控制。
OBJECTIVE To establish a high performance capillary electrophoresis (HPCE) for simultaneous determination of aconitine, hypaconitine, mesaconitine, benzoyl aconitine, benzoyl aconitine and benzoyl New aconitine alkali content of the method. Methods 40 mmol·L-1 sodium acetate-60 mmol·L-1 ammonium acetate-80 mmol·L-1tris reagent-25% methanol (pH 8.3) was used as a buffer solution without standard fused silica capillary (75 μm × 64.5 cm and an effective length of 56 cm). The separation voltage was 16 kV, the detection wavelength was 235 nm, the capillary temperature was 25 ℃ and the pressure was 5 kPa × 6 s. Results The linear relationship between the concentration of six alkaloids and the peak area was good (r> 0.999 7). The recoveries were 95.25% -103.91%. Using this method, the content of six alkaloids in aconite medicinal materials was determined and satisfactory results were obtained. Conclusion The method is simple, accurate and reproducible. It can be used to evaluate and control the intrinsic quality of Aconite.