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目的以等量血吸虫尾蚴分别进行1次性和分2次、3次、4次感染4组小鼠,建立小鼠血吸虫感染模型,观察不同感染频次对肝纤维化程度的影响。方法通过肝脾指数来判断肝损害程度;化学发光法检测血清中透明质酸(HA);病理切片HE染色观察胶原沉积、纤维形成情况和形成率。结果 1次性感染组肝纤维化形成率及纤维化程度不高,肝损害较轻;2次以上感染组,18周后所有小鼠均有肝脏纤维化的形成,纤维化程度与感染频次呈正相关,肝损害程度随感染频次增加而加重。结论血吸虫反复感染可导致患者严重肝损害,患者一旦被血吸虫尾蚴感染应及时治疗,避免反复多次感染。
Objective To infect Schistosoma japonicum with Schistosoma japonicum in the same dose of 1, 2, 3 and 4 times respectively to establish a mouse model of schistosomiasis infection and observe the effect of different frequency of infection on the degree of hepatic fibrosis. Methods Hepatic spleen index was used to determine the degree of hepatic injury. Hyaluronan (HA) was detected by chemiluminescence method. The collagen deposition, fiber formation and formation rate were observed with HE staining. Results The rate of formation and fibrosis of liver fibrosis in one-time infection group was not high, and the liver damage was lighter. In the group of infection more than 2 times, all the mice had hepatic fibrosis after 18 weeks, and the degree of fibrosis and infection frequency were positive Related, the degree of liver damage increases with the frequency of infection. Conclusion Schistosoma infection can lead to severe liver damage in patients with repeated infection of schistosome cercariae should be promptly treated to avoid repeated infections.