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液体培养蛹虫草鲁山菌株,以菌丝体产量为指标,对培养基的碳源、氮源、无机盐、维生素B1进行单因素试验,并在单因素基础上进行四因素三水平正交试验,结果表明:影响液体培养菌丝产量的主次因素依次为氮源、碳源、维生素B1和无机盐。最佳培养基为:蔗糖20 g/L、蛋白胨25 g/L、维生素B10.15 g/L,无机盐为KH2PO41.5 g/L+MgSO40.5 g/L+CaCl20.25 g/L。
Liquid culture of Cordyceps militaris Lushan strain mycelium yield as an indicator of the medium of carbon, nitrogen, inorganic salts, vitamin B1 single factor test, and on the basis of single factor four-factor three-level orthogonal test The results showed that the primary and secondary factors affecting the mycelial yield of liquid culture were nitrogen source, carbon source, vitamin B1 and inorganic salt. The optimum culture medium was sucrose 20 g / L, peptone 25 g / L, vitamin B10.15 g / L and inorganic salts KH2PO41.5 g / L + MgSO40.5 g / L + CaCl20.25 g / L.