AIM To investigate the expression and clinical pathological significance of ROR2 and WNT5 a in gallbladder squamous/adenosquamous carcinoma(SC/ASC) and adenocarcinoma(AC). METHODS En Vision immunohistochemistry was used to stain for ROR2 and WNT5 a in 46 SC/ASC patients and 80 AC patients. RESULTS Poorly differentiated AC among AC patients aged > 45 years were significantly more frequent compared with SC/ASC patients, while tumors with a maximal diameter > 3 cm in the SC/ASC group were significantly more frequent compared with the AC group. Positive ROR2 and WNT5 a expression was significantly lower in SC/ASC or AC with a maximal mass diameter ≤ 3 cm, a TNM stage of?Ⅰ?+ Ⅱ, no lymph node metastasis, no surrounding invasion, and radical resection than in patients with a maximal mass diameter > 3 cm, TNM stage Ⅳ, lymph node metastasis, surrounding invasion, and no resection. Positive ROR2 expression in patients with highly differentiated SC/ASC was significantly lower than in patients with poorly differentiated SC/ASC. Positive ROR2 and WNT5 a expression levels in highly differentiated AC were significantly lower than in poorly differentiated AC. Kaplan-Meier survival analysis showed that differentiation degree, maximal mass diameter, TNM stage, lymph node metastasis, surrounding invasion, surgical procedure and the ROR2 and WNT5 a expression levels were closely related to average survival of SC/ASC or AC. The survival of SC/ASC or AC patients with positive expression of ROR2 and WNT5 a was significantly shorter than that of patients with negative expression results. Cox multivariate analysis revealed that poor differentiation, a maximal diameter of the mass ≥ 3 cm, TNM stage Ⅲ or Ⅳ, lymph node metastasis, surrounding invasion, unresected surgery and positive ROR2 or WNT5 a expression in the SC/ASC or AC patients were negatively correlated with the postoperative survival rate and positively correlated with mortality, which are risk factors and independent prognostic predictors. CONCLUSION SC/ASC or AC patients with positive ROR2 or WNT5 a expression generally have a poor prognosis. AIM To investigate the expression and clinical pathological significance of ROR2 and WNT5 a in gallbladder squamous / adenosquamous carcinoma (SC / ASC) and adenocarcinoma (AC). METHODS En Vision immunohistochemistry was used to stain for ROR2 and WNT5 a in 46 SC / ASC patients and 80 AC patients. RESULTS Poorly differentiated AC among AC patients aged> 45 years were significantly more frequent than with SC / ASC patients, while tumors with a maximal diameter> 3 cm in the SC / ASC group were significantly more frequent than with the AC group. Positive ROR2 and WNT5 a expression was significantly lower in SC / ASC or AC with a maximal mass diameter ≤ 3 cm, a TNM stage of? Ⅰ? + II, no lymph node metastasis, no surrounding invasion, and radical resection than in Positive ROR2 expression in patients with highly differentiated SC / ASC was significantly lower than in pati, with a maximal mass diameter> 3 cm, TNM stage Ⅳ, lymph node metastasis, surrounding invasion, and no resection. Positive ROR2 and WNT5 a expression levels in highly differentiated AC were significantly lower than in poorly differentiated AC. Kaplan-Meier survival analysis showed that differentiation degree, maximal mass diameter, TNM stage, lymph node metastasis, surrounding invasion, surgical procedure and the ROR2 and WNT5 a expression levels were closely related to the average survival of SC / ASC or AC. The survival of SC / ASC or AC patients with positive expression of ROR2 and WNT5 a was significantly shorter than that of patients with negative expression results. Cox multivariate analysis revealed that poor differentiation, a maximal diameter of the mass ≥ 3 cm, TNM stage III or IV, lymph node metastasis, surrounding invasion, unresected surgery and positive ROR2 or WNT5 a expression in the SC / ASC or AC patients were negatively correlated with the postoperative survival rate and positively correlated with mortality, which are risk factors and independent prognostic predictors. CONCLUSION SC / ASC or AC patients with positive ROR2 or WNT5 a expression generally have a poor prognosis.