论文部分内容阅读
改良的人嗜碱细胞脱颗粒试验是一种观察人嗜碱细胞对特异性变应原脱颗粒反应的简易方法。其步骤包括聚蔗糖——泛影葡胺浓缩嗜碱细胞,用阿尔新蓝特异性染色细胞浆内肝素颗粒,借助于血球计数板计数未脱粒细胞,以加入特异性变应原后染色细胞减少30％以上判定为阳性。本文以蒿属花粉作为特异性变应原,对50例由蒿属花粉过敏所致的典型秋季花粉症患者作嗜碱细胞脱颗粒试验。结果发现,在花粉症缓解期阳性率为72％,且其阳性率随蒿属花粉皮试反应强度的增加而升高,而在花粉症急性发作期阳性率为34％,明显低于缓解期。由此对“嗜碱细胞释放能力”这一重要参数进行了初步探讨,认为在花粉症急性发作期嗜碱细胞在体内已经或正在和特异性变应原发生作用,此时如果嗜碱细胞在体外再和变应原接触时,由于其释放能力的限制,将处于相对的低反应状态。30例正常人的阴性反应结果则排除了蒿属花粉变应原的非特异性脱颗粒作用。本研究表明该方法在花粉变态反应研究中应用的敏感性和稳定性。
The modified human basophil degranulation assay is a simple method for observing human basophil degranulation of specific allergens. The steps include enriching the basophils with Ficoll-diatrizoate, staining intracellularly heparin particles with Alzheimer’s, counting non-degranulating cells by means of a hemocytometer, reducing the number of stained cells after addition of the specific allergen 30% or more judged as positive. In this paper, artemisia pollen as a specific allergen, 50 cases of Artemisia pollen allergy caused by typical fall hay fever patients as basophil degranulation test. The results showed that the positive rate in remission of hay fever was 72%, and its positive rate increased with the increase of the reaction intensity of the pollen skin test, while the positive rate was 34% in the acute attack of hay fever, which was significantly lower than that in the remission . Based on this, the important parameter of “basophil release capacity” was preliminarily discussed, and it was considered that the basophil cells had already been or were in the body with specific allergens during the acute attack of hay fever. In vitro and then allergen contact, due to its release capacity constraints, will be in a relatively low reaction state. Negative results of 30 normal individuals ruled out non-specific degranulation of the artemisia pollen allergen. This study shows the sensitivity and stability of this method in pollen allergy studies.