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目的：观察球囊损伤对动脉平滑肌细胞（ＳＭＣ）表皮生长因子受体（ＥＧＦＲ）表达的影响，探讨ＥＧＦＲ与再狭窄的关系。方法：于兔右颈总动脉球囊损伤前及损伤后２４ｈ、４８ｈ、７２ｈ、７天和１４天，取实验动脉段用免疫组化ＬＳＡＢ法检测ＥＧＦＲ的表达。结果：正常颈总动脉中膜未见ＥＧＦＲ阳性表达，动脉球囊损伤后２４ｈ中膜部分ＳＭＣ显示ＥＧＦＲ阳性表达，４８ｈ中膜呈ＥＧＦＲ阳性表达的ＳＭＣ较２４ｈ增加，７２ｈ又较４８ｈ进一步增加；７天和１４天动脉中膜ＥＧＦＲ阳性表达的ＳＭＣ均较７２ｈ显著减少，但新生内膜中增殖的ＳＭＣ显示出大量的ＥＧＦＲ阳性表达。阳性染色主要定位于ＳＭＣ的胞膜。结论：球囊损伤使动脉ＥＧＦＲ表达显著增加，ＥＧＦＲ与再狭窄形成过程中ＳＭＣ增殖有关。
OBJECTIVE: To observe the effect of balloon injury on the expression of epidermal growth factor receptor (EGFR) in arterial smooth muscle cells (SMC) and to explore the relationship between EGFR and restenosis. Methods: The experimental arterial segments were examined by immunohistochemistry (LSAB) before and at the right carotid artery balloon injury and at 24h, 48h, 72h, 7d and 14d after injury. Results: The expression of EGFR in normal carotid artery was not observed in normal carotid artery. The expression of EGFR in SMMC of middle part of carotid artery 24 hours after arterial balloon injury was higher than that in 24h and 48 hours after injury. The SMC of positive expression of EGFR on day and on day 14 was significantly lower than that on 72h, but the proliferation of SMC on neointimal showed a large number of positive EGFR expression. Positive staining mainly located in the cell membrane of SMC. CONCLUSION: Balloon injury significantly increases the expression of EGFR in arteries and EGFR is related to the proliferation of SMC during restenosis.